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Methods Mol Biol. 2013;1012:65-76. doi: 10.1007/978-1-62703-429-6_5.

Detection of c-Myc protein-protein interactions and phosphorylation status by immunoprecipitation.

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  • 1Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, OR, USA.

Abstract

Co-immunoprecipitation is an invaluable technique in evaluating native protein-protein interactions in vitro and in vivo. However, it can be difficult to detect interactions of a very transient nature, particularly interactions with phosphatases and kinases. The evaluation of the phosphorylation status of c-Myc can also be challenging with the current commercially available phosphorylation sensitive antibodies. Here, we describe two protocols: one for the co-immunoprecipitation of endogenous c-Myc to detect protein-protein interactions and second, for the immunoprecipitation of endogenous c-Myc to probe for phosphorylation status.

PMID:
24006058
[PubMed - indexed for MEDLINE]

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