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J Evol Biol. 2013 Oct;26(10):2123-34. doi: 10.1111/jeb.12233. Epub 2013 Sep 4.

Fold or hold: experimental evolution in vitro.

Author information

  • 1Ashworth Laboratories, Institute of Evolutionary Biology, University of Edinburgh, Edinburgh, UK.

Abstract

We introduce a system for experimental evolution consisting of populations of short oligonucleotides (Oli populations) evolving in a modified quantitative polymerase chain reaction (qPCR). It is tractable at the genetic, genomic, phenotypic and fitness levels. The Oli system uses DNA hairpins designed to form structures that self-prime under defined conditions. Selection acts on the phenotype of self-priming, after which differences in fitness are amplified and quantified using qPCR. We outline the methodological and bioinformatics tools for the Oli system here and demonstrate that it can be used as a conventional experimental evolution model system by test-driving it in an experiment investigating adaptive evolution under different rates of environmental change.

© 2013 The Authors. Journal of Evolutionary Biology © 2013 European Society For Evolutionary Biology.

KEYWORDS:

DNA hairpin; adaptation; aptamer; experimental evolution; polymerase chain reaction

PMID:
24003997
[PubMed - indexed for MEDLINE]
PMCID:
PMC4274015
Free PMC Article
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