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Biosens Bioelectron. 2014 Jan 15;51:304-9. doi: 10.1016/j.bios.2013.07.055. Epub 2013 Aug 6.

Proximity-dependent isothermal cycle amplification for small-molecule detection based on surface enhanced Raman scattering.

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  • 1Key Laboratory of Biochemical Analysis, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.

Abstract

A novel proximity-dependent isothermal cycle amplification (PDICA) strategy has been proposed and successfully used for the determination of cocaine coupled with surface enhanced Raman scattering (SERS). For enhancing the SERS signal, Raman dye molecules modified bio-barcode DNA and gold nanoparticles (AuNPs) are used to prepare the Raman probes. Magnetic beads (MBs) are used as the carrier of amplification template and signal output products for circumventing the problem of high background induced by excess bio-barcode DNA. In the presence of target molecules, two label-free proximity probes can hybridize with each other and subsequently opens the hairpin connector-probe to perform the PDICA reaction including the target recycling amplification and strand-displacement amplification. As a result, abundant AuNPs Raman probes can be anchored on the surface of MBs and a low detection limit of 0.1 nM for cocaine is obtained. This assay also exhibits an excellent selectivity and has been successfully performed in human serum, which confirms the reliability and practicality of this protocol.

© 2013 Elsevier B.V. All rights reserved.

KEYWORDS:

Aptamer; Cocaine; Dual recognition; Proximity-dependent isothermal cycle amplification; Surface-enhanced Raman scattering

PMID:
23994277
[PubMed - indexed for MEDLINE]
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