Format

Send to:

Choose Destination
See comment in PubMed Commons below
J Allergy Clin Immunol. 2014 Feb;133(2):543-50. doi: 10.1016/j.jaci.2013.06.036. Epub 2013 Aug 24.

Maternal and cord blood miR-223 expression associates with prenatal tobacco smoke exposure and low regulatory T-cell numbers.

Author information

  • 1Department of Environmental Immunology, UFZ-Helmholtz Centre for Environmental Research Leipzig, Leipzig, Germany. Electronic address: gunda.herberth@ufz.de.
  • 2Department of Environmental Immunology, UFZ-Helmholtz Centre for Environmental Research Leipzig, Leipzig, Germany.
  • 3Core Facility Studies, UFZ-Helmholtz Centre for Environmental Research Leipzig, Leipzig, Germany.
  • 4Ivana Tuerbachova Laboratory for Epigenetics, Epiontis GmbH, Berlin, Germany.
  • 5Department of Metabolomics, UFZ-Helmholtz Centre for Environmental Research Leipzig, Leipzig, Germany.
  • 6Department of Metabolomics, UFZ-Helmholtz Centre for Environmental Research Leipzig, Leipzig, Germany; Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Aalborg, Denmark.
  • 7Institute for Clinical Immunology, University of Leipzig, Leipzig, Germany.
  • 8Children's Hospital, Municipal Hospital "St Georg," Academic Teaching Hospital of the University of Leipzig, Leipzig, Germany.
  • 9Department of Environmental Immunology, UFZ-Helmholtz Centre for Environmental Research Leipzig, Leipzig, Germany. Electronic address: irina.lehmann@ufz.de.

Abstract

BACKGROUND:

There is evidence that microRNAs (miRNAs) are sensitive to environmental stressors, including tobacco smoke. On the other hand, miRNAs are involved in immune regulation, such as regulatory T (Treg) cell differentiation. The aim of the present study was to investigate the association between prenatal tobacco smoke exposure, miRNAs, and Treg cell numbers.

METHODS:

Within a prospective mother-child study (Lifestyle and Environmental Factors and Their Influence on Newborns Allergy Risk), we analyzed the expression of miR-155 and miR-223 together with Treg cell numbers in maternal blood during pregnancy, as well as in cord blood (n = 441). Tobacco smoke exposure was assessed based on questionnaire answers and maternal urine cotinine levels. Additionally, the concentration of smoking-related volatile organic compounds was measured in dwellings of study participants.

RESULTS:

Both maternal and cord blood miR-223 expressions were positively correlated with maternal urine cotinine levels. An association was also found between maternal miR-223 expression and indoor concentrations of benzene and toluene. High miR-223 expression was associated with lower Treg cell numbers in maternal and cord blood. Furthermore, children with lower Treg cell numbers at birth had a higher risk of atopic dermatitis during the first 3 years of life. The concentration of the toluene metabolite S-benzylmercapturic acid in maternal urine was associated with decreased cord blood, but not maternal blood, miR-155 expression. A relationship between miR-155 expression and Treg cell numbers was not found.

CONCLUSIONS:

For the first time, we show that maternal tobacco smoke exposure during pregnancy correlates with the level of miRNA-223 expression in blood, with an effect on children's cord blood Treg cell numbers and subsequent allergy risk.

Copyright © 2013 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.

KEYWORDS:

ETS; Effector T; Environmental tobacco smoke; FOXP3; Forkhead box protein 3; Genomic DNA; LINA; Lifestyle and Environmental Factors and Their Influence on Newborns Allergy Risk; MR; Mean ratio; MicroRNA; RT; Regulatory T; Reverse transcription; S-benzylmercapturic acid; S-phenylmercapturic acid; SBMA; SPMA; TSDR; Teff; Treg; Treg cell–specific demethylated region; VOC; Volatile organic compound; cord blood; gDNA; miR-155; miR-223; miRNA; pregnancy; regulatory T cells; tobacco smoke

PMID:
23978443
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk