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J Lipid Res. 2013 Nov;54(11):3215-9. doi: 10.1194/jlr.D042226. Epub 2013 Aug 21.

Preparation of 20-HETE using multifunctional enzyme type 2-negative Starmerella bombicola.

Author information

  • 1Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.

Abstract

The metabolism of arachidonic acid (ARA) by cytochrome P450 ω/ω-1-hydroxylases leads to the formation of 20-hydroxyeicosatetraenoic acid (20-HETE), which is an important lipid-signaling molecule involved in regulation of vascular tone, angiogenesis, and inflammation. Development of a simple method to prepare 20-HETE would greatly facilitate the investigation of its biological activities. The nonpathogenic yeast Starmerella bombicola has been shown to convert exogenously added arachidonic acid to 20-HETE via the biosynthetic pathway of sophorolipids; however, the yield was low. Here we demonstrate that genetic knockout of multifunctional enzyme type 2 (MFE-2), which is involved in the β-oxidation of fatty acids, significantly increases the yield of ARA conversion to 20-HETE and allows practical preparation of 20-HETE.

KEYWORDS:

20-hydroxyeicosatetraenoic acid; arachidonic acid; sophorolipids

PMID:
23966667
[PubMed - indexed for MEDLINE]
PMCID:
PMC3793626
Free PMC Article
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