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Methods Mol Biol. 2013;1035:25-33. doi: 10.1007/978-1-62703-508-8_3.

Visualization of adult stem cells within their niches using the Drosophila germline as a model system.

Author information

  • 1Max Planck Research Group of Gene Expression and Signaling, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.

Abstract

The germaria of the fruit fly Drosophila melanogaster present an excellent model to study germline stem cell-niche interactions. Two to three adult stem cells are surrounded by a number of somatic cells that form the niche. Here we describe how Drosophilae germaria can be dissected and specifically immuno-stained to allow for identification and analysis of both the adult stem cells and their somatic niche cells.

PMID:
23959979
[PubMed - indexed for MEDLINE]

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