Format

Send to:

Choose Destination
See comment in PubMed Commons below
Hum Vaccin Immunother. 2013 Dec;9(12):2578-83. doi: 10.4161/hv.26096. Epub 2013 Aug 16.

Infection-enhancing lipopeptides do not improve intranasal immunization of cotton rats with a delta-G candidate live-attenuated human respiratory syncytial virus vaccine.

Author information

  • 1Department of Viroscience; Erasmus MC; Rotterdam, The Netherlands.
  • 2National Institute of Public Health and the Environment; Bilthoven, The Netherlands.
  • 3Department of Viroscience; Erasmus MC; Rotterdam, The Netherlands; National Institute of Public Health and the Environment; Bilthoven, The Netherlands.

Abstract

Development of live-attenuated human respiratory syncytial virus (HRSV) vaccines has proven to be difficult. Several vaccine candidates were found to be over-attenuated and displayed limited immunogenicity. Recently, we identified three synthetic cationic lipopeptides that enhanced paramyxovirus infections in vitro. The infection enhancement proved to be mediated by enhanced virus binding to target cells. We hypothesized that these lipopeptides can be used as adjuvants to promote immune responses induced by live-attenuated paramyxovirus vaccines. This hypothesis was tested in a vaccination and challenge model in cotton rats, using a previously described recombinant live-attenuated candidate HRSV vaccine lacking the gene encoding the G glycoprotein (rHRSVΔG). Surprisingly, intranasal vaccination of cotton rats with rHRSVΔG formulated in infection-enhancing lipopeptides resulted in reduced virus loads in nasopharyngeal lavages, reduced seroconversion levels and reduced protection from wild-type HRSV challenge. In conclusion, we were unable to demonstrate the feasibility of lipopeptides as adjuvants for a candidate live-attenuated HRSV vaccine in the cotton rat model.

KEYWORDS:

HRSV; adjuvant; lipopeptide; live-attenuated virus vaccine; paramyxovirus

PMID:
23955280
[PubMed - indexed for MEDLINE]
PMCID:
PMC4162065
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Landes Bioscience Icon for PubMed Central
    Loading ...
    Write to the Help Desk