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Transplant Proc. 2013 Jul-Aug;45(6):2553-8. doi: 10.1016/j.transproceed.2013.03.045.

Small interfering RNA targeting nuclear factor kappa B to prevent vein graft stenosis in rat models.

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  • 1Department of Cardiovascular Surgery, QiLu Hospital, Shandong University, Jinan, Shandong Province, China.

Abstract

BACKGROUND:

Intimal hyperplasia plays an important role in vein graft stenosis. Inflammatory injury, especially nuclear factor kappaB (NF-κB) gene activation, is highly involved in stenosis progression. We examined whether neointimal hyperplasia and vein graft stenosis could be inhibited by silencing the NF-κB gene with small interference RNA (siRNA).

METHODS:

Sixty adult male Sprague-Dawley rats were randomly divided into a normal vein group, a vein graft group, a scrambled siRNA group, and an NF-κB siRNA group. We performed reverse interpositional grafting of the autologous external jugular vein to the abdominal aorta. Vein grafts were treated with liposome and gel complexes containing NF-κB siRNA or scrambled siRNA. The levels of monocyte chemoattractant protein -1, tumor necrosis factor-α, and NF-κB p65 in vessel tissues were evaluated after surgery for content of proliferating cell nuclear antigen (PCNA) and vascular wall thickness.

RESULTS:

NF-κB siRNA treated vein graft showed less neointimal formation and fewer positive PCNA cells (P < .05). In addition there were lower levels of, NF-κB p65 protein and of inflammatory mediators (P < .05) compared with the vein graft group.

CONCLUSION:

Our study suggested that siRNA transfection suppressed NF-κB expression, reduced inflammatory factors, lessened neointimal proliferation, and suppressed PCNA.

Copyright © 2013 Elsevier Inc. All rights reserved.

PMID:
23953580
[PubMed - indexed for MEDLINE]
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