The mechanism of antifungal action of a new polyene macrolide antibiotic antifungalmycin 702 from Streptomyces padanus JAU4234 on the rice sheath blight pathogen Rhizoctonia solani

Zhi-Qiang Xiong; Xiao-Rong Tu; Sai-Jin Wei; Lin Huang; Xun-Hang Li; Hui Lu; Guo-Quan Tu

doi:10.1371/journal.pone.0073884

The mechanism of antifungal action of a new polyene macrolide antibiotic antifungalmycin 702 from Streptomyces padanus JAU4234 on the rice sheath blight pathogen Rhizoctonia solani

PLoS One. 2013 Aug 12;8(8):e73884. doi: 10.1371/journal.pone.0073884. eCollection 2013.

Authors

Zhi-Qiang Xiong¹, Xiao-Rong Tu, Sai-Jin Wei, Lin Huang, Xun-Hang Li, Hui Lu, Guo-Quan Tu

Affiliation

¹ Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China. zhqxiong@sibs.ac.cn

Abstract

Antifungalmycin 702, a new polyene macrolide antibiotic produced by Streptomycespadanus JAU4234, has a broad antifungal activity and may have potential future agricultural and/or clinical applications. However, the mechanism of antifungal action of antifungalmycin 702 remains unknown. Antifungalmycin 702 strongly inhibited mycelial growth and sclerotia formation/germination of Rhizoctonia solani. When treated with antifungalmycin 702, the hyphae morphology of R. solani became more irregular. The membrane and the cellular organelles were disrupted and there were many vacuoles in the cellular space. The lesion in the plasma membrane was detected through the increase of membrane permeability, lipid peroxidation and leakage of cell constituents. In summary, antifungalmycin 702 may exert its antifungal activity against R. solani by changing the structure of cell membranes and the cytoskeleton and interacting with the organelles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / biosynthesis
Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology
Antifungal Agents / chemistry
Antifungal Agents / metabolism
Antifungal Agents / pharmacology*
Cell Membrane / drug effects
Cell Membrane / metabolism
Dose-Response Relationship, Drug
Hyphae / cytology
Hyphae / drug effects
Hyphae / ultrastructure
Macrolides / metabolism
Macrolides / pharmacology
Microbial Sensitivity Tests
Oryza / microbiology*
Permeability / drug effects
Plant Diseases / microbiology
Polyenes / metabolism
Polyenes / pharmacology
Rhizoctonia / drug effects*
Rhizoctonia / growth & development
Rhizoctonia / ultrastructure
Streptomyces / metabolism*

Substances

Anti-Bacterial Agents
Antifungal Agents
Macrolides
Polyenes

Grants and funding

This work was supported by the National Natural Science Foundation of China (grants no. 30960011 and 31071724), Natural Science Foundation of Jiangxi Province, China (grants no. 2009GZN0030 and 2010GZN0037) and Key Technology R&D Program of Jiangxi Province, China (grant no. 2007BN14002). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.