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Chem Commun (Camb). 2013 Oct 4;49(76):8546-8. doi: 10.1039/c3cc44986f.

In vitro monitoring of the formation of pentamers from the monomer of GST fused HPV 16 L1.

Author information

  • 1State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun, 130012, China. yqwu@jlu.edu.cn.

Abstract

The recombinant GST fusion protein HPV 16 L1 from E. coli was proved to exist as a monomer rather than a pentamer, providing the possibility of real-time monitoring of the pentamer formation in vitro. Time-dependent kinetic studies of the process were performed for the first time by using static light scattering and western blot analysis, where the essential factors were revealed, offering a new biotechnical approach for virus control and/or the development of anti-viral agents.

PMID:
23945594
[PubMed - indexed for MEDLINE]
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