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Nat Methods. 2013 Sep;10(9):869-71. doi: 10.1038/nmeth.2601. Epub 2013 Aug 11.

Allele-specific detection of single mRNA molecules in situ.

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  • 1Harvard University Graduate Biophysics Program, Harvard Medical School, Boston, Massachusetts, USA.

Abstract

We describe a method for fluorescence in situ identification of individual mRNA molecules, allowing quantitative and accurate measurement, in single cells, of allele-specific transcripts that differ by only a few nucleotides. By using a combination of allele-specific and non-allele-specific probe libraries, we achieve >95% detection accuracy. We investigate the allele-specific stochastic expression of Nanog, which encodes a pluripotency factor, in murine embryonic stem cells.

PMID:
23934076
[PubMed - indexed for MEDLINE]
PMCID:
PMC3789122
Free PMC Article
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