Abstract
By comparing 16S rDNA cloning and 454 pyrosequencing in the Nephrops norvegicus midgut, several common bacterial OTUs were detected. However, when only one method is to be selected, it needs to be considered whether the revealing of rare OTUs or their accurate phylogenetic relationships is mostly preferred.
Keywords:
16S rRNA; Bacteria; Diversity; Gut; Nephrops norvegicus; Pyrosequencing.
Copyright © 2013 Elsevier Ltd. All rights reserved.
MeSH terms
-
Animals
-
Bacteria / classification*
-
Bacteria / genetics*
-
Biota*
-
Cluster Analysis
-
DNA, Bacterial / chemistry
-
DNA, Bacterial / genetics
-
DNA, Ribosomal / chemistry
-
DNA, Ribosomal / genetics
-
Gastrointestinal Tract / microbiology
-
Molecular Sequence Data
-
Nephropidae / microbiology*
-
Phylogeny
-
RNA, Ribosomal, 16S / genetics
-
Sequence Analysis, DNA
Substances
-
DNA, Bacterial
-
DNA, Ribosomal
-
RNA, Ribosomal, 16S
Associated data
-
GENBANK/GQ866066
-
GENBANK/GQ866067
-
GENBANK/GQ866068
-
GENBANK/GQ866069
-
GENBANK/GQ866070
-
GENBANK/GQ866071
-
GENBANK/GQ866072
-
GENBANK/GQ866073
-
GENBANK/GQ866074
-
GENBANK/GQ866075
-
GENBANK/GQ866076
-
GENBANK/GQ866077
-
GENBANK/GQ866078
-
GENBANK/GQ866079
-
GENBANK/GQ866080
-
GENBANK/GQ866081
-
GENBANK/GQ866082
-
GENBANK/GQ866083
-
GENBANK/GQ866084
-
GENBANK/GQ866085
-
GENBANK/GQ866086
-
GENBANK/GQ866087
-
GENBANK/GQ866088
-
GENBANK/GQ866089
-
GENBANK/GQ866090
-
GENBANK/GQ866091
-
GENBANK/GQ866092
-
GENBANK/GQ866093
-
GENBANK/GQ866094
-
GENBANK/GQ866095
-
GENBANK/GQ866096
-
GENBANK/GQ866097
-
GENBANK/GQ866098
-
GENBANK/GQ866099
-
GENBANK/GQ866100
-
GENBANK/GQ866101
-
GENBANK/GQ866102
-
GENBANK/GQ866103
-
GENBANK/GQ866104
-
GENBANK/GQ866105
-
GENBANK/GQ866106
-
GENBANK/GQ866107
-
GENBANK/GQ866108
-
GENBANK/GQ866109
-
GENBANK/GQ866110
-
GENBANK/GQ866111