Display Settings:

Format

Send to:

Choose Destination
Enzyme Microb Technol. 2013 Sep 10;53(4):271-7. doi: 10.1016/j.enzmictec.2013.05.002. Epub 2013 May 23.

Kinetic study of a novel thermo-stable α-carbonic anhydrase for biomimetic CO2 capture.

Author information

  • 1Istituto di Ricerche sulla Combustione - Consiglio Nazionale delle Ricerche, Napoli, Italy.

Abstract

Biomimetic CO2 capture includes environmentally friendly solutions based on carbonic anhydrase (CA), an enzyme that increases CO2 absorption rate in conventional acid-gas scrubbing processes. The present contribution reports the characterization of a new recombinant carbonic anhydrase, SspCA, isolated from the thermophile bacterium Sulphurhydrogenibium yellowstonense sp. YO3AOP1. The kinetics of SspCA was characterized in terms of first order CO2 hydration rate according to a procedure based on CO2 absorption tests in a stirred cell apparatus. The first order kinetic constant at 25°C was 9.16 × 10(6) L/(mols). An appropriate investigation on SspCA stability was carried out to assess its long-term resistance to high temperatures as in all capture processes based on absorption/vacuum-desorption cycles. Its half-life was 53 and 8 days at 40 °C and 70 °C, respectively.

Copyright © 2013 Elsevier Inc. All rights reserved.

KEYWORDS:

Absorption; CO(2) capture; Carbonic anhydrase; Thermo-stable enzyme

PMID:
23931693
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk