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Methods Cell Biol. 2013;114:99-123. doi: 10.1016/B978-0-12-407761-4.00005-1.

Fluorescent protein applications in microscopy.

Author information

  • 1Department of Biochemistry, Stanford Medical School, Stanford University, Stanford, California, USA.

Abstract

The use of fluorescent proteins (FPs) in modern cell biology and microscopy has had an extraordinary impact on our ability to investigate dynamic processes in living cells. FPs are unique in that fluorescence is encoded solely by the primary amino acid sequence of the FP and does not require enzymatic modification or cofactors. This genetically encoded fluorescence enables the expression of FPs in diverse cells and organisms and the detection of that fluorescence in living systems. This chapter focuses on microscopy-based applications of FP detection to monitor protein localization, dynamics, interaction, and the cellular environment.

Copyright © 2013 Elsevier Inc. All rights reserved.

KEYWORDS:

Chromophore; Fluorescent proteins; GFP; Photoactivation; Protein dynamics; Protein localization; Time-lapse imaging

PMID:
23931504
[PubMed - indexed for MEDLINE]
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