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Pulm Pharmacol Ther. 2014 Apr;27(2):150-5. doi: 10.1016/j.pupt.2013.07.007. Epub 2013 Aug 3.

Protective effect of resveratrol against endotoxemia-induced lung injury involves the reduction of oxidative/nitrative stress.

Author information

  • 1Department of Physiology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China; Department of Respiration, Kongjiang Hospital, Shanghai 200093, China.
  • 2The Eight-year Program on Clinical Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
  • 3Department of Physiology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China.
  • 4Department of Physiology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China. Electronic address: xiaoyanzhu@smmu.edu.cn.
  • 5Department of Pathophysiology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China. Electronic address: liuyujianz@yahoo.com.cn.

Abstract

BACKGROUND:

Resveratrol, a natural plant polyphenol, has received increasing attention because its varied bioactivities, including the inhibition of tumorigenesis, lipid modification and calorie-restriction. We aimed to investigate the effect of resveratrol on oxidative/nitrative stress in endotoxemia-associated acute lung injury.

METHODS:

Mice were injected with lipopolysaccharide (LPS, 5 mg/kg, ip). Resveratrol at a dose of 0.3 mg/kg was administered alone or immediately before injection of LPS. Twenty four hours later, lung tissues were collected for histopathologic examination, and determination of malondialdehyde (MDA), H2O2, reduced/oxidized glutathione (GSH/GSSG) ratio, total antioxidant capacity (T-AOC), superoxide dismutase (SOD) activity, catalase (CAT) activity, inducible nitric oxide synthase (iNOS) expression, nitric oxide (NO) and peroxynitrite production.

RESULTS:

Resveratrol treatment improves histopathological changes in the lung during endotoxemia. Increased oxidative stress in endotoxemic lung was reversed by resveratrol treatment, as evidenced by the decreases of pro-oxidant biomarker (MDA and H2O2), and the increases of anti-oxidant biomarkers (GSH/GSSG ratio, T-AOC, CAT and SOD activity). Treatment with resveratrol inhibited endotoxemia-induced iNOS expression and NO production. Moreover, peroxynitrite formation in endotoxemic lung was significantly attenuated after resveratrol treatment.

CONCLUSIONS:

Resveratrol exerts protective effects against acute endotoxemia-associated lung injury. These beneficial effects may be due to both the anti-oxidant and anti-nitrative properties of resveratrol. These findings support the potential for resveratrol as a possible pharmacological agent to reduce acute lung injury resulting from oxidative/nitrative damage.

Copyright © 2013 Elsevier Ltd. All rights reserved.

KEYWORDS:

Endotoxemia; Lung; Nitrative stress; Oxidative stress; Resveratrol

PMID:
23921197
[PubMed - in process]
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