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Nat Methods. 2013 Sep;10(9):865-7. doi: 10.1038/nmeth.2589. Epub 2013 Aug 4.

Visualizing SNVs to quantify allele-specific expression in single cells.

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  • 1Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA. MCMartin@lbl.gov

Abstract

We present a FISH-based method for detecting single-nucleotide variants (SNVs) in exons and introns on individual RNA transcripts with high efficiency. We used this method to quantify allelic expression in cell populations and in single cells, and also to distinguish maternal from paternal chromosomes in single cells.

PMID:
23913259
[PubMed - indexed for MEDLINE]
PMCID:
PMC3771873
Free PMC Article
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