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Mol Immunol. 2013 Dec;56(4):488-96. doi: 10.1016/j.molimm.2013.06.015. Epub 2013 Aug 1.

Immature mouse granulocytic myeloid cells are characterized by production of ficolin-B.

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  • 1Institute of Immunology, University of Regensburg, Regensburg, Germany.

Erratum in

  • Mol Immunol. 2015 Jan;99(1):604.


Ficolins activate the lectin pathway of the complement system upon binding to carbohydrate patterns on pathogens. To characterize the producer cells of ficolin-B the expression of mouse ficolin-B, the orthologue of human M-ficolin, was studied in macrophages and dendritic cells during differentiation from bone marrow cells, in primary granulocytes, and during differentiation of granulocytes derived from ER-Hoxb8 cells. Expression of ficolin-B mRNA declined in all myeloid cell types to low levels during terminal differentiation. However, in contrast to macrophages and dendritic cells, ficolin-B expression was enhanced upon activation in granulocytes. High expression of ficolin-B was observed in primary immature neutrophilic CD11b(+) Ly-6C(int) Ly-6G(high) granulocytes when isolated from the bone marrow, in particular during sepsis. Ficolin-B was demonstrated in lysates of primary granulocytes, ER-Hoxb8-derived granulocytes, bone marrow-derived macrophages, and dendritic cells. Native ficolin-B from cell lysates and supernatants of granulocytes activated the lectin pathway as measured by binding to MASP-2 and inducing C4 deposition. Specific staining demonstrated intra-cellular or cell associated ficolin-B protein in activated immature granulocytes deposited in a granular fashion. This study shows that ficolin-B is stored in and set free from immature granulocytic myeloid cells indicating a role in the early infection-induced cellular response of these inflammatory cells.

Copyright © 2013 Elsevier Ltd. All rights reserved.


BMDC; BMDM; CLP; Complement activation; FcnB; GlcNAc; GlcNAc-BSA; Inflammatory cells; Lectin pathway; MASP; MBL; MBL-associated serine protease; MDSC; N-acetyl glucosamine; N-acetyl glucosamine-coupled BSA; NET; PEC; PMN; bone marrow-derived dendritic cells; bone marrow-derived macrophages; cecal ligation and puncture; ficolin-B; mannan-binding lectin; myeloid cell-derived suppressor cells; neutrophil extracellular trap; peritoneal exudate cells; polymorphonuclear neutrophils

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