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Gut Liver. 2013 Jul;7(4):411-6. doi: 10.5009/gnl.2013.7.4.411. Epub 2013 Jun 11.

Proteomic identification of proteins suggestive of immune-mediated response or neuronal degeneration in serum of achalasia patients.

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  • 1Department of Gastroenterology, Ajou University School of Medicine, Suwon, Korea.

Abstract

BACKGROUND/AIMS:

The primary pathophysiologic abnormality in achalasia is known to be a loss of inhibitory myenteric ganglion cells, which may result from an immune-mediated response or neuronal degeneration. The aim of this study was to identify proteins suggestive of an immune-mediated response or neuronal degeneration in the serum of achalasia patients using a proteomic analysis.

METHODS:

Blood samples were collected from five symptomatic achalasia patients and five sex- and age-matched healthy controls. Serum proteomic analysis was conducted, and the protein spots were identified using matrix-assisted laser desorption ionization/time-of-flight and a proteomics analyzer. The serum level of C3 was measured by enzyme-linked immunosorbent assay in nine patients with achalasia and 18 sex- and age-matched healthy controls.

RESULTS:

Of the 658 matched protein spots, 28 spots were up-regulated over 2-fold in the serum from achalasia patients compared with that from controls. The up-regulated proteins included complement C4B5, complement C3, cyclin-dependent kinase 5, transthyretin, and alpha 2 macroglobulin. The serum levels of C3 in achalasia patients were significantly higher than those of controls.

CONCLUSIONS:

The serum proteomic analysis of achalasia patients suggests an immune-mediated response or neuronal degeneration. Further validation studies in larger samples and the esophageal tissue of achalasia patients are required.

KEYWORDS:

Esophageal achalasia; Immune response; Neuronal degeneration; Proteomics

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