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Prostaglandins Leukot Essent Fatty Acids. 2013 Sep;89(4):221-5. doi: 10.1016/j.plefa.2013.07.001. Epub 2013 Jul 23.

The effect of omega-3 polyunsaturated fatty acids on the inflammatory response of the amnion.

Author information

  • 1University of Edinburgh/MRC Centre for Reproductive Health, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh EH16 4TJ, UK. Electronic address: L.Frew@sms.ed.ac.uk.

Abstract

OBJECTIVE:

To investigate the effect of omega-3 PUFAs, eicosapentanoic acid (EPA) and docosohexanoic acid (DHA) on inflammatory cytokine production in the amnion.

STUDY DESIGN:

Amnion explants were obtained at elective caesarean sections and cultured in vitro with EPA and DHA. IL-8 and IL-6 secretion was determined by ELISA, the role of PPARγ was investigated using specific agonists and antagonists and activity of MMP assessed by gelatin zymography.

RESULTS:

A combination of EPA and DHA significantly reduced the concentration of IL-8 and IL-6 released into the supernatant compared to untreated controls (p<0.001). Stimulation of PPARγ with troglitazone reduced IL-8 production, and the PPARγ antagonist GW9662 partially reversed this effect. The activity of MMP-9 was also significantly reduced by treatment with EPA and DHA in combination compared to untreated control (p<0.05).

CONCLUSION:

The omega-3 PUFAs EPA and DHA decrease the inflammatory response of the amnion, and this may be partially mediated through PPARγ.

© 2013 Elsevier Ltd. All rights reserved.

KEYWORDS:

Foetal membranes; Inflammation; PPARγ; Polyunsaturated fatty acids; Pregnancy

PMID:
23890848
[PubMed - indexed for MEDLINE]
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