Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
J Endocrinol Invest. 2013 Dec;36(11):1055-61. doi: 10.3275/9063. Epub 2013 Jul 23.

Follicular-derived neoplasms: morphometric and genetic differences.

Author information

  • 1Dipartimento di Patologia Chirurgica, Medica, Molecolare e dell'Area Critica, Universit├á di Pisa, Via Paradisa 2, 56124 Pisa, Italy.

Abstract

BACKGROUND:

The distinction between follicular adenomas (FAs) and well differentiated follicular and papillary carcinomas is often a demanding task and sometimes only intuitive.

AIM:

We report an histomorphological evaluation of follicular neoplasms [FAs, follicular carcinomas (FCs), and follicular variant of papillary carcinomas (FVPTCs)], supported by a qualitative and quantitative image analysis and by a molecular characterization.

MATERIAL AND METHODS:

Tumor fibrosis and haemorrhage, neoplastic capsule thickness, follicle diameter, number of neoplastic cells, nuclear diameter of neoplastic cells, vessels density, vessels area and intratumoral distribution were evaluated. Ras and BRAF mutations, RET/PTC1, RET/PTC3, and PAX8/PPAR╬│ rearrangements were analyzed. Correlations with clinico-pathological features have been studied.

RESULTS:

We found that FAs had a more extensive intratumoral haemorrhage, while malignant neoplasms were characterized by an evident fibrosis, higher cellularity and larger size. FVPTCs had higher nuclear diameter; cells count was higher in the minimally invasive follicular thyroid carcinomas, as well as a thickener neoplastic capsule. The CD34 stain showed a higher microvessel density in the FVPTCs group. A higher peripheral vessels distribution was observed only in malignant neoplasms. We observed overall Ras mutations in 2.4% of adenomas, in 41.5% of FVPTCs, and in 44.8% of FCs. It is outstanding that there is a marked difference in the Ras mutation distribution between the benign and malignant tumors in our series.

CONCLUSIONS:

We found that genotyping of Ras gene family together with an accurate analysis of selected morphological features could help in the differential diagnosis of follicular-derived thyroid neoplasms.

PMID:
23888303
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk