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Magn Reson Med. 2014 Jun;71(6):2051-8. doi: 10.1002/mrm.24871. Epub 2013 Jul 22.

Dynamic metabolic imaging of hyperpolarized [2-(13) C]pyruvate using spiral chemical shift imaging with alternating spectral band excitation.

Author information

  • 1SRI International, Neuroscience Program, Menlo Park, California, USA; Department of Radiology, Stanford University, Lucas MRI Center, Stanford, California, USA.

Abstract

PURPOSE:

In contrast to [1-(13) C]pyruvate, hyperpolarized [2-(13) C]pyruvate permits the ability to follow the (13) C label beyond flux through pyruvate dehydrogenase complex and investigate the incorporation of acetyl-coenzyme A into different metabolic pathways. However, chemical shift imaging (CSI) with [2-(13) C]pyruvate is challenging owing to the large spectral dispersion of the resonances, which also leads to severe chemical shift displacement artifacts for slice-selective acquisitions.

METHODS:

This study introduces a sequence for three-dimensional CSI of [2-(13) C]pyruvate using spectrally selective excitation of limited frequency bands containing a subset of metabolites. Dynamic CSI data were acquired alternately from multiple frequency bands in phantoms for sequence testing and in vivo in rat heart.

RESULTS:

Phantom experiments verified the radiofrequency pulse design and demonstrated that the signal behavior of each group of resonances was unaffected by excitation of the other frequency bands. Dynamic three-dimensional (13) C CSI data demonstrated the sequence capability to image pyruvate, lactate, acetylcarnitine, glutamate, and acetoacetate, enabling the analysis of organ-specific spectra and metabolite time courses.

CONCLUSIONS:

The presented method allows CSI of widely separated resonances without chemical shift displacement artifact, acquiring multiple frequency bands alternately to obtain dynamic time-course information. This approach enables robust imaging of downstream metabolic products of acetyl-coenzyme A with hyperpolarized [2-(13) C]pyruvate.

Copyright © 2013 Wiley Periodicals, Inc.

KEYWORDS:

[2-13C]pyruvate; dynamic metabolic imaging; hyperpolarized 13C; spiral CSI

PMID:
23878057
[PubMed - indexed for MEDLINE]
PMCID:
PMC3849119
Free PMC Article
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