Send to:

Choose Destination
See comment in PubMed Commons below
J Chromatogr A. 2013 Aug 23;1304:127-32. doi: 10.1016/j.chroma.2013.06.078. Epub 2013 Jul 4.

Reversed phase ion-pairing chromatography of an oligolysine mixture in different mobile phases: effort of searching critical chromatography conditions.

Author information

  • 1Center for Biocatalysis and Bioprocessing of Macromolecules, The Polytechnic Institute of New York University, Six Metrotech Center, Brooklyn, NY 11201, USA.


Our earlier study [J. Chromatogr. A 1218 (2011) 7765] on separation of an oligolysine mixture consisting of chains with 2-8 lysine residues (number of lysine residues, dp=2-8) by ion-pairing reversed-phase chromatography using heptafluorobutyric acid (HFBA) as an ion pairing reagent at fixed mobile phase acetonitrile (ACN) content was extended to isocratic elution conditions with different ACN percentages. The present work explored how manipulating the mobile phase HFBA concentration ([HFBA]) and %-ACN content influences separations of the oligolysine mixture. The closed pairing model was used to analyze variation of the retention factor as a function of [HFBA]. The partition coefficient of the paired peptide decreased with increasing %-ACN. Pairing of HFBA to oligolysine was cooperative, and the effect increased when %-ACN in the mobile phase was lowered. A plot of the partition coefficient as a function of %-ACN for oligolysines varying in dp converged at one ACN content, indicating a critical condition in which components of different dp co-elute.

Copyright © 2013 Elsevier B.V. All rights reserved.


Critical chromatography condition; Heptafluorobutyric acid; Ion-pairing chromatography; Mobile phase composition; Oligolysine

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk