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J Microbiol Methods. 2013 Sep;94(3):300-2. doi: 10.1016/j.mimet.2013.07.008. Epub 2013 Jul 20.

Rapid genotyping of three polymorphisms in the LBP gene using a triplex pyrosequencing approach.

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  • 1State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, China.

Abstract

We described a triplex polymerase chain reaction (PCR) and triplex pyrosequencing assay which allowed a simultaneous determination of three tag single nuleotide polymorphisms (tag SNPs) in the lipopolysaccharide-binding protein (LBP) gene: rs1780623, rs11536972 and rs2232618. This method enables a fast and cost-effective genotyping and a simultaneous determination of the three tag SNPs.

© 2013.

KEYWORDS:

Lipopolysaccharide-Binding Protein; Single Nuleotide Polymorphism; Triplex pyrosequencing

PMID:
23876431
[PubMed - indexed for MEDLINE]
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