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Food Chem. 2013 Dec 1;141(3):1796-806. doi: 10.1016/j.foodchem.2013.04.104. Epub 2013 May 9.

Simultaneous determination of eighteen steroid hormones in antler velvet by gas chromatography-tandem mass spectrometry.

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  • 1College of Chemistry, Jilin University, Changchun 130012, PR China.

Abstract

A method for the simultaneous determination of eighteen steroid hormones, including 17α-ethinylestradiol, 17α-estradiol, 19-nortestosterone, estriol, testosterone, androsterone, 17β-estradiol, estrone, progesterone, 17α-hydroxyprogesterone, medroxyprogesterone, norethisterone acetate, testosterone 17-propionate, medroxyprogesterone-17-acetate, corticosterone, megestrol-17-acetate, chlromadinone-17-acetate and 17β-estradiol-benzoate, in antler velvet was developed by solid-phase extraction (SPE) and gas chromatography-tandem mass spectrometry (GC-MS/MS). Prior to the determination by GC-MS/MS, the analytes were derivatized with heptafluorobutyric acid anhydrides (HFBA). The recoveries were in the range of 62.13-104.00% for eighteen compounds at concentration levels (5-500 μg/L) and relative standard deviations (RSDs) were lower than 17.15%. The detection limits were from 0.2 to 22.3 μg/kg for the steroid hormones. The developed method was rapid, sensitive and successfully applied to the determination of steroid hormones in antler velvet samples collected from three countries.

Copyright © 2013 Elsevier Ltd. All rights reserved.

KEYWORDS:

Antler velvet; Determination; Gas chromatography–mass spectrometry/mass spectrometry; Steroid hormones

PMID:
23870893
[PubMed - indexed for MEDLINE]
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