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Philos Mag (Abingdon). 2012 Jul 1;92(19-21):2648-2661.

Biomolecular membrane protein crystallization.

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  • 1Department of Chemistry, Iowa State University, Ames, IA 50011, USA.

Abstract

Integral membrane proteins comprise approximately 30% of the sequenced genomes, and there is an immediate need for their high-resolution structural information. Currently, the most reliable approach to obtain these structures is x-ray crystallography. However, obtaining crystals of membrane proteins that diffract to high resolution appears to be quite challenging, and remains a major obstacle in structural determination. This brief review summarizes a variety of methodologies for use in crystallizing these membrane proteins. Hopefully, by introducing the available methods, techniques, and providing a general understanding of membrane proteins, a rational decision can be made about now to crystallize these complex materials.

PMID:
23869195
[PubMed]
PMCID:
PMC3712538
Free PMC Article
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