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Transgenic Res. 2013 Dec;22(6):1107-18. doi: 10.1007/s11248-013-9729-0. Epub 2013 Jul 16.

Pig transgenesis by piggyBac transposition in combination with somatic cell nuclear transfer.

Author information

  • 1Department of Animal Genetics, Breeding and Reproduction, South China Agricultural University, The New Building of College of Animal Science, Room 315, Guangzhou, 510642, Guangdong, People's Republic of China.

Abstract

The production of animals by somatic cell nuclear transfer (SCNT) is inefficient, with approximately 2% of micromanipulated oocytes going to term and resulting in live births. However, it is the most commonly used method for the generation of cloned transgenic livestock as it facilitates the attainment of transgenic animals once the nuclear donor cells are stably transfected and more importantly as alternatives methods of transgenesis in farm animals have proven even less efficient. Here we describe piggyBac-mediated transposition of a transgene into porcine primary cells and use of these genetically modified cells as nuclear donors for the generation of transgenic pigs by SCNT. Gene transfer by piggyBac transposition serves to provide an alternative approach for the transfection of nuclear donor cells used in SCNT.

PMID:
23857557
[PubMed - indexed for MEDLINE]
PMCID:
PMC3838457
[Available on 2014/12/1]
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