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Stem Cells Transl Med. 2013 Aug;2(8):558-66. doi: 10.5966/sctm.2013-0006. Epub 2013 Jul 11.

Blood cell-derived induced pluripotent stem cells free of reprogramming factors generated by Sendai viral vectors.

Author information

  • 1Department of Medicine and Institute for Human Genetics, University of California, San Francisco, San Francisco, CA 94143, USA. lin.ye@ucsf.edu

Abstract

The discovery of induced pluripotent stem cells (iPSCs) holds great promise for regenerative medicine since it is possible to produce patient-specific pluripotent stem cells from affected individuals for potential autologous treatment. Using nonintegrating cytoplasmic Sendai viral vectors, we generated iPSCs efficiently from adult mobilized CD34⁺ and peripheral blood mononuclear cells. After 5-8 passages, the Sendai viral genome could not be detected by real-time quantitative reverse transcription-polymerase chain reaction. Using the spin embryoid body method, we showed that these blood cell-derived iPSCs could efficiently be differentiated into hematopoietic stem and progenitor cells without the need of coculture with either mouse or human stromal cells. We obtained up to 40% CD34⁺ of which ~25% were CD34⁺/CD43⁺ hematopoietic precursors that could readily be differentiated into mature blood cells. Our study demonstrated a reproducible protocol for reprogramming blood cells into transgene-free iPSCs by the Sendai viral vector method. Maintenance of the genomic integrity of iPSCs without integration of exogenous DNA should allow the development of therapeutic-grade stem cells for regenerative medicine.

KEYWORDS:

CD34+; Differentiation; Hematopoietic progenitors; Reprogramming

PMID:
23847002
[PubMed - indexed for MEDLINE]
PMCID:
PMC3726135
Free PMC Article
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