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Curr Protoc Cytom. 2013 Jul;Chapter 12:Unit 12.30. doi: 10.1002/0471142956.cy1230s65.

A review of reagents for fluorescence microscopy of cellular compartments and structures, part I: BacMam labeling and reagents for vesicular structures.

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  • 1Molecular Probes Labeling and Detection, Life Technologies, Eugene, OR, USA.

Abstract

Fluorescent labeling of vesicular structures in cultured cells, particularly for live cells, can be challenging for a number of reasons. The first challenge is to identify a reagent that will be specific enough where some structures have a number of potential reagents and others very few options. The emergence of BacMam constructs has allowed more easy-to-use choices. Presented here is a discussion of BacMam constructs as well as a review of commercially-available reagents for labeling vesicular structures in cells, including endosomes, peroxisomes, lysosomes, and autophagosomes, complete with a featured reagent for each structure, recommended protocol, troubleshooting guide, and example image.

© 2013 by John Wiley & Sons, Inc.

PMID:
23835803
[PubMed - indexed for MEDLINE]
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