N-terminus determination: FAD and NADP binding domain mapping of hog liver flavin-containing monooxygenase by tandem mass spectrometry

Biochem Biophys Res Commun. 1990 Jul 31;170(2):937-43. doi: 10.1016/0006-291x(90)92181-x.

Abstract

Highly purified hog liver flavin-containing monooxygenase was sequentially denatured, reduced, carboxymethylated, and digested with endoproteinase Glu-C. The purified peptides were subjected to mass spectrometric analysis and the amino acid sequence of selected fragments was determined by tandem mass spectrometry. The amino acid sequence of the first 12 residues of the N-terminus was: Ac-Ala-Lys-Arg-Val-Ala-Ile-Val-Gly-Ala-Gly-Val-Ser-Gly. The amino acid sequence determined for another peptide was: Lys-Ser-Val-Leu-Val-Val-Gly-Met-Gly-Asn-Ser-Gly-Thr-Asp-Ile-Ala-Val-Glu. The results provide direct evidence for the structure of the N-terminal modification of the protein and for the existence of the FAD and NADP binding domains of Gly-X-Gly-X-X-Gly.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetylation
  • Amino Acid Sequence
  • Animals
  • Chromatography, High Pressure Liquid
  • Flavin-Adenine Dinucleotide / metabolism*
  • Liver / enzymology*
  • Mass Spectrometry
  • Molecular Sequence Data
  • NADP / metabolism*
  • Oxygenases / metabolism*
  • Swine

Substances

  • Flavin-Adenine Dinucleotide
  • NADP
  • Oxygenases
  • dimethylaniline monooxygenase (N-oxide forming)