Display Settings:

Format

Send to:

Choose Destination
Mater Sci Eng C Mater Biol Appl. 2013 Apr 1;33(3):1554-60. doi: 10.1016/j.msec.2012.12.061. Epub 2012 Dec 23.

Mg ion implantation on SLA-treated titanium surface and its effects on the behavior of mesenchymal stem cell.

Author information

  • 1Wonkwang Bone Regeneration Research Institute, Wonkwang University, Iksan 570-749, Republic of Korea.

Abstract

Magnesium (Mg) is one of the most important ions associated with bone osseointegration. The aim of this study was to evaluate the cellular effects of Mg implantation in titanium (Ti) surfaces treated with sand blast using large grit and acid etching (SLA). Mg ions were implanted into the surface via vacuum arc source ion implantation. The surface morphology, chemical properties, and the amount of Mg ion release were evaluated by scanning electron microscopy (SEM), Auger electron spectroscopy (AES), Rutherford backscattering spectroscopy (RBS), and inductively coupled plasma-optical emission spectrometer (ICP-OES). Human mesenchymal stem cells (hMSCs) were used to evaluate cellular parameters such as proliferation, cytotoxicity, and adhesion morphology by MTS assay, live/dead assay, and SEM. Furthermore, osteoblast differentiation was determined on the basis of alkaline phosphatase (ALP) activity and the degree of calcium accumulation. In the Mg ion-implanted disk, 2.3×10(16) ions/cm(2) was retained. However, after Mg ion implantation, the surface morphology did not change. Implanted Mg ions were rapidly released during the first 7 days in vitro. The MTS assay, live/dead assay, and SEM demonstrated increased cell attachment and growth on the Mg ion-implanted surface. In particular, Mg ion implantation increased the initial cell adhesion, and in an osteoblast differentiation assay, ALP activity and calcium accumulation. These findings suggest that Mg ion implantation using the plasma source ion implantation (PSII) technique may be useful for SLA-treated Ti dental implants to improve their osseointegration capacity.

Copyright © 2012 Elsevier B.V. All rights reserved.

KEYWORDS:

Magnesium; Mesenchymal stem cells; Mg ion implanted titanium implant treated with sand blast with large grit and acid etching; Mg-SLA Ti; PSII; SLA; SLA Ti; Surface modification; Titanium implant; Vacuum arc source ion implantation; plasma source ion implantation; sand blast with large grit and acid etching; titanium implant treated with sand blast with large grit and acid etching

PMID:
23827608
[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk