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J Clin Microbiol. 2013 Sep;51(9):3110-2. doi: 10.1128/JCM.01455-13. Epub 2013 Jul 3.

Rapid differentiation of cryptic species within Cryptococcus gattii by a duplex PCR assay.

Author information

  • 1Medical Mycology Laboratory, Department of Dermatology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine.

Abstract

Cryptococcus gattii consists of four cryptic species, VGI, VGII, VGIII, and VGIV. Herein, a duplex PCR assay using two primer pairs targeting the vacuolar membrane gene and the intergenic spacer region was developed. It successfully distinguished the cryptic species according to the distinct size of the amplicons.

PMID:
23824773
[PubMed - indexed for MEDLINE]
PMCID:
PMC3754660
Free PMC Article

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