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J Sci Food Agric. 2014 Feb;94(3):576-84. doi: 10.1002/jsfa.6298. Epub 2013 Jul 31.

Increase of the adhesion ability and display of a rumen fungal xylanase on the cell surface of Lactobacillus casei by using a listerial cell-wall-anchoring protein.

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  • 1Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan.

Abstract

BACKGROUND:

Lactobacillus, which has great adhesion ability to intestinal mucosa and is able to hydrolyse plant cell walls, can be used more efficiently as a feed additive. To increase the adhesion ability and display a fungal xylanase on the cell surface of Lactobacillus casei, the Listeria monocytogenes cell-wall-anchoring protein gene, mub, was introduced into L. casei ATCC 393 cells and used as a fusion partner to display the rumen fungal xylanase XynCDBFV on the cell surface of the transformed strains.

RESULTS:

The transformed strain L. casei pNZ-mub, which harboured mub gene, displayed recombinant Mub on its cell surface and showed greater adhesion ability to Caco-2 cells than the parental strain. The transformed strain L. casei pNZ-mub/xyn, which harboured mub-xynCDBFV fusion gene, acquired the capacity to break down oat spelt xylan and exhibited greater competition ability against the adhesion of L. monocytogenes to Caco-2 cells, in comparison with the parental strain.

CONCLUSION:

Mub has a potential to be used as a fusion partner to display heterologous proteins on the cell surface of Lactobacillus. Moreover, this is the first report of the successful display of xylanase on the cell surface of Lactobacillus.

© 2013 Society of Chemical Industry.

KEYWORDS:

Lactobacillus casei; Listeria monocytogenes; cell-surface display; cell-wall-anchoring protein; xylanase

PMID:
23824609
[PubMed - indexed for MEDLINE]
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