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J Steroid Biochem Mol Biol. 2013 Nov;138:325-33. doi: 10.1016/j.jsbmb.2013.06.005. Epub 2013 Jun 28.

Effects of granulosa cells on steroidogenesis, proliferation and apoptosis of stromal cells and theca cells derived from the goat ovary.

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  • 1College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address:


The aim of this study was to investigate the effect of granulosa cells from small antral follicles on steroidogenesis, proliferation and apoptosis of goat ovarian stromal and theca cells in vitro. Using Transwell co-culture system, we evaluated androgen production, LH responsiveness, cell proliferation and apoptosis and some molecular expression regarding steroidogenic enzyme and apoptosis-related genes in stromal and theca cells. The results indicated that the co-culture with granulosa cells increased steroidogenesis, LH responsiveness and bcl-2 gene expression as well as decreased apoptotic bax and bad expressions in stromal and theca cells. Thus, granulosa cells had a capacity of promoting steroidogenesis in stromal cell and LH responsiveness in cortical stromal cells, maintaining steroidogenesis in theca cells, inhibiting apoptosis of cortical stromal cells and improving anti-apoptotic abilities of stromal and theca cells.

Copyright © 2013 Elsevier Ltd. All rights reserved.


17alpha-hydroxylase/C17, 20-lyase; 3beta-hydroxysteroid deydrogenase/isomerase; Apoptosis; B-cell lymphoma protein 2; BCL-2-associated X protein; BCL-2-associated agonist of cell death; Bad; Bax; Bcl-2; CYP11A1; CYP17A1; Cell proliferation; Cholesterol side-chain cleavage cytochrome P450; GAPDH; GCs; Goat; Granulosa cells; HSD3B1; LHR; Ovarian stromal cells; SCs; SMs; STAR; Steroidogenesis; TCs; Theca cells; glyceraldehyde-3-phosphate dehydrogenase; granulosa cells; luteinizing hormone receptor; ovarian cortical stromal cells; ovarian medullary stromal cells; steroidogenic acute regulatory protein; theca cells

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