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J Microbiol Methods. 2013 Sep;94(3):213-6. doi: 10.1016/j.mimet.2013.06.019. Epub 2013 Jun 28.

Rapid assay for detecting gyrA and parC mutations associated with fluoroquinolone resistance in Enterobacteriaceae.

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  • 1Department of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan.


We developed a PCR-RFLP assay to detect mutations in the quinolone-resistance determining regions of gyrA and parC associated with fluoroquinolone resistance in Enterobacteriaceae. The assay detected mutations associated with reduced susceptibility to fluoroquinolones and may therefore serve as a specific, rapid, inexpensive, and simple testing alternative.

© 2013.


Enterobacteriaceae; Fluoroquinolones; PCR-restriction fragment length polymorphism; Quinolone resistance-determining regions

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