Format

Send to

Choose Destination
See comment in PubMed Commons below
Muscle Nerve. 2013 Aug;48(2):286-92. doi: 10.1002/mus.23760. Epub 2013 Jun 29.

Sarcomere lengths in human extensor carpi radialis brevis measured by microendoscopy.

Author information

  • 1Department of Mechanical Engineering, Stanford University, Stanford, California, USA.

Abstract

INTRODUCTION:

Second-harmonic generation microendoscopy is a minimally invasive technique to image sarcomeres and measure their lengths in humans, but motion artifact and low signal have limited the use of this novel technique.

METHODS:

We discovered that an excitation wavelength of 960 nm maximized image signal; this enabled an image acquisition rate of 3 frames/s, which decreased motion artifact. We then used microendoscopy to measure sarcomere lengths in the human extensor carpi radialis brevis with the wrist at 45° extension and 45° flexion in 7 subjects. We also measured the variability in sarcomere lengths within single fibers.

RESULTS:

Average sarcomere lengths in 45° extension were 2.93±0.29 μm (±SD) and increased to 3.58±0.19 μm in 45° flexion. Within single fibers the standard deviation of sarcomere lengths in series was 0.20 μm.

CONCLUSIONS:

Microendoscopy can be used to measure sarcomere lengths at different body postures. Lengths of sarcomeres in series within a fiber vary substantially.

© 2013 Wiley Periodicals, Inc.

KEYWORDS:

clinical microendoscopy; dye-free in vivo imaging; human microendoscopy; human wrist biomechanics; in vivo two photon microscopy; multiphoton imaging; multiphoton microendoscopy; muscle architecture; muscle fiber length; sarcomere inhomogeneity; sarcomere length variability; sarcomere operating length; second-harmonic generation microendoscopy; skeletal muscle

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk