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Biosens Bioelectron. 2013 Nov 15;49:457-61. doi: 10.1016/j.bios.2013.06.006. Epub 2013 Jun 11.

One-step signal amplified lateral flow strip biosensor for ultrasensitive and on-site detection of bisphenol A (BPA) in aqueous samples.

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  • 1School of Biotechnology and Food Engineering, Anhui Provincial Key Lab of Advanced Functional Materials & Devices, Hefei University of Technology, Hefei 230009, PR China.

Abstract

A one-step signal amplified lateral flow strip (LFS) biosensor has been developed for ultrasensitive and on-site visual detection of bisphenol A (BPA). This signal amplified LFS was based on the dual labeling using different-sized gold nanoparticles (Duo-LFS). This Duo-LFS could achieve BPA detection with 0.5 ng/mL as the visual sensitivity by naked eye observation and with 0.076 ng/mL as the limit of detection (LOD) for semi-quantitative detection by software analysis, which is at least 10-fold improvement of the sensitivity of traditional LFS based methods. This one-step signal amplified lateral flow strip biosensor and related signal enhancement method could be adopted as a potential generous technique for all LFS-based detection methods.

Crown Copyright © 2013. Published by Elsevier B.V. All rights reserved.

KEYWORDS:

BPA detection; Food safety; Lateral flow strip; Sensitivity amplification

PMID:
23811478
[PubMed - indexed for MEDLINE]
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