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J Cutan Pathol. 2013 Sep;40(9):807-11. doi: 10.1111/cup.12189. Epub 2013 Jun 29.

Comparison of virtual microscopy and glass slide microscopy among dermatology residents during a simulated in-training examination.

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  • 1Department of Dermatology, Mayo Clinic, Rochester, MN 55905, USA.



Virtual microscopy is increasingly being used in dermatopathology educational settings.


The objective is to evaluate diagnostic accuracy and attitudes between virtual microscopy and traditional glass slide microscopy among dermatology residents.


A 48-question dermatopathology examination was administered to 35 dermatology residents at three different dermatology residency training sites during the 2011-2012 academic year with half (n = 24) of the questions using the gold standard of glass slide microscopy and half (n = 24) using whole, scanned virtual slides. Correct number of questions using glass slides and virtual slides was evaluated. Participants were surveyed regarding previous experience with digital slide imaging; quality, ease of use, and speed of slide review; and overall microscopy preferences.


Overall, diagnostic accuracy was better with glass slides than virtual slides (p = 0.01). However, no statistically significant difference was found in diagnostic accuracy of first-year trainees (p > 0.99) or trainees with exposure to virtual microscopy greater than two times per month (p = 0.27). There was no overall personal preference for glass slide vs. virtual microscopy.


Different cases and questions were used for glass slides and virtual microscopy.


Diagnostic accuracy with virtual microscopy is dependent on year of residency training and prior experience with virtual microscopy.

© 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.


dermatology; pathology; residency

[PubMed - indexed for MEDLINE]
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