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PLoS One. 2013 Jun 14;8(6):e66748. doi: 10.1371/journal.pone.0066748. Print 2013.

Intraventricular injection of human dental pulp stem cells improves hypoxic-ischemic brain damage in neonatal rats.

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  • 1Department of Pediatrics, Xiangya Hospital, Central South University, Changsha, China.

Abstract

OBJECTIVE:

To investigate the effect of intraventricular injection of human dental pulp stem cells (DPSCs) on hypoxic-ischemic brain damage (HIBD) in neonatal rats.

METHODS:

Thirty-six neonatal rats (postnatal day 7) were assigned to control, HIBD, or HIBD+DPSC groups (n = 12 each group). For induction of HIBD, rats underwent left carotid artery ligation and were exposed to 8% to 10% oxygen for 2 h. Hoechst 33324-labeled human DPSCs were injected into the left lateral ventricle 3 days after HIBD. Behavioral assays were performed to assess hypoxic-ischemic encephalopathy (HIE), and on postnatal day 45, DPSC survival was assessed and expression of neural and glial markers was evaluated by immunohistochemistry and Western blot.

RESULTS:

The HIBD group showed significant deficiencies compared to control on T-maze, radial water maze, and postural reflex tests, and the HIBD+DPSC group showed significant improvement on all behavioral tests. On postnatal day 45, Hoechst 33324-labeled DPSC nuclei were visible in the injected region and left cortex. Subsets of DPSCs showed immunostaining for neuronal (neuron-specific enolase [NSE], Nestin) and glial markers (glial fibrillary acidic protein [GFAP], O4). Significantly decreased staining/expression for NSE, GFAP, and O4 was found in the HBID group compared to control, and this was significantly increased in the HBID+DPSC group.

CONCLUSION:

Intraventricular injection of human DPSCs improves HIBD in neonatal rats.

PMID:
23799131
[PubMed - indexed for MEDLINE]
PMCID:
PMC3682969
Free PMC Article
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