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Zhongguo Gu Shang. 2013 Mar;26(3):232-5.

[Effects of geniposide on SNP-induced apoptosis of chondrocyte and cell cycle].

[Article in Chinese]

Author information

  • 1Department of Orthopaedics, China Three Gorges University, Yichang Central People's Hospital, yichang, Hubei, China.

Abstract

OBJECTIVE:

To study the effects of Geniposide on SNP(sodium nitroprusside)-induced apoptosis of chondrocyte in vitro and cell cycle.

METHODS:

The chondrocyte of three-week-old SD rats were separated and cultivated. The second generation of chondrocyte cells were involved in experiment. Chondrocyte proliferation was measured by assay; flow cytometer were adopted to observe cell cycle and apoptosis rate; NO examination adopted nitrate reductase method.

RESULTS:

Geniposide could significantly decrease the percentage of SNP-induced chondrocytes in G0/G1 phase and increased percentage in S phase and G2/M phase. The apoptosis of chondrocyte and the concentration of NO in the culture supernatants was reduced significantly (r=0.917, P<0.01).

CONCLUSION:

Geniposide could impact SNP-induced apoptosis of chondrocyte by reducing the concentration of NO in the culture supernatants, promoting proliferation of chondrocytes, which is a probable and important mechanism of Geniposide preventing osteoarthritis.

PMID:
23795444
[PubMed - indexed for MEDLINE]
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