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Genome editing in human pluripotent stem cells.


StemBook [Internet]. Cambridge (MA): Harvard Stem Cell Institute; 2008-.
2013 Apr 29.


Genome editing is used to make targeted modifications to the genome of eukaryotic cells. There are many potential applications of genome editing in human pluripotent stem cells (hPSCs) including the generation of knockout and reporter cell lines. This protocol describes a system for efficient genome editing in hPSCs using engineered transcription activator-like effector nucleases (TALENs) or clustered regularly interspaced short palindromic repeat (CRISPR) technology.

Copyright: © 2013 Derek T. Peters, Chad A. Cowan and Kiran Musunuru.

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