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Macromol Biosci. 2013 Aug;13(8):1048-58. doi: 10.1002/mabi.201300044. Epub 2013 Jun 17.

The influence of matrix degradation and functionality on cell survival and morphogenesis in PEG-based hydrogels.

Author information

  • 1Department of Biomedical Engineering, Purdue School of Engineering and Technology, Indiana University-Purdue University at Indianapolis, Indianapolis, IN 46202, USA.

Abstract

Two norbornene-functionalized PEG macromers are synthesized to render hydrogels with different hydrolytic degradability. Dithiol-containing linkers such as dithiothreitol or biscysteine-containing peptides are used to control proteolytic degradability. The influence of thiol-ene gel degradability on cell survival and morphogenesis in 3D is assessed using hMSCs and pancreatic MIN6 β cells. The initial cell viability can be negatively affected in highly crosslinked thiol-ene hydrogels. When cells are encapsulated in thiol-ene gels lacking cell-adhesive motifs, their survival and proliferation are promoted in more hydrolytically labile hydrogels. The degree of 3D cell spreading in encapsulated hMSCs is enhanced when the matrices are immobilized with cell-adhesive motifs.

© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

KEYWORDS:

cell/material interactions; degradation; hydrogels; mesenchymal stem cells

PMID:
23776086
[PubMed - indexed for MEDLINE]
PMCID:
PMC3819142
Free PMC Article

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