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APMIS. 2014 Feb;122(2):155-60. doi: 10.1111/apm.12121. Epub 2013 Jun 12.

Evaluation of the rapid loop-mediated isothermal amplification assay Illumigene for diagnosis of Clostridium difficile in an outbreak situation.

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  • 1Department of Laboratory Medicine, Microbiology, Örebro University Hospital, Örebro, Sweden.


An outbreak of Clostridium difficile infection (CDI) at Höglandet Hospital Eksjö in southern Sweden in 2011 was mainly due to a multidrug-resistant PCR ribotype 046 (30% of all samples). Diagnostics used routinely was the Vidas CDAB assay, but to control the outbreak the rapid loop-mediated isothermal amplification (LAMP) assay Illumigene was introduced and both techniques were compared to Toxigenic culture (TC) prospectively. The LAMP assay had a superior sensitivity, that is, 98% compared to 79% for the Vidas CDAB assay. Most importantly, the mean turn-around-time from collecting sample to result was reduced from 59 h to 2 h enabling early isolation of patients and effective hygiene precautions. This may potentially decrease the morbidity and nosocomial transmissions of C. difficile.

© 2013 The Authors. APMIS published by John Wiley & Sons Ltd.


Clostridium difficile; Illumigene; LAMP; Vidas CDAB; diagnostics

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