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Elife. 2013 Jun 4;2:e00704. doi: 10.7554/eLife.00704.

MCU encodes the pore conducting mitochondrial calcium currents.

Author information

  • 1Cardiovascular Research Center , Massachusetts General Hospital , Boston , United States ; Department of Cardiology , Howard Hughes Medical Institute, Boston Children's Hospital , Boston , United States.

Abstract

Mitochondrial calcium (Ca(2+)) import is a well-described phenomenon regulating cell survival and ATP production. Of multiple pathways allowing such entry, the mitochondrial Ca(2+) uniporter is a highly Ca(2+)-selective channel complex encoded by several recently-discovered genes. However, the identity of the pore-forming subunit remains to be established, since knockdown of all the candidate uniporter genes inhibit Ca(2+) uptake in imaging assays, and reconstitution experiments have been equivocal. To definitively identify the channel, we use whole-mitoplast voltage-clamping, the technique that originally established the uniporter as a Ca(2+) channel. We show that RNAi-mediated knockdown of the mitochondrial calcium uniporter (MCU) gene reduces mitochondrial Ca(2+) current (I MiCa ), whereas overexpression increases it. Additionally, a classic feature of I MiCa , its sensitivity to ruthenium red inhibition, can be abolished by a point mutation in the putative pore domain without altering current magnitude. These analyses establish that MCU encodes the pore-forming subunit of the uniporter channel. DOI:http://dx.doi.org/10.7554/eLife.00704.001.

KEYWORDS:

Human; MCUR1; MICU1; calcium channel; electrophysiology; mitoplast; ruthenium red

PMID:
23755363
[PubMed - indexed for MEDLINE]
PMCID:
PMC3673318
Free PMC Article
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