Display Settings:

Format

Send to:

Choose Destination
Int J Biochem Cell Biol. 2013 Aug;45(8):1825-32. doi: 10.1016/j.biocel.2013.06.003. Epub 2013 Jun 8.

Early growth response 2 (Egr2) plays opposing roles in committing C3H10T1/2 stem cells to adipocytes and smooth muscle-like cells.

Author information

  • 1Key Laboratory of Molecular Medicine, The Ministry of Education, Department of Biochemistry and Molecular Biology, Fudan University Shanghai Medical College, Shanghai 200032, PR China.

Abstract

Early growth response 2 (Egr2) is a zinc-finger transcription factor that acts as an important modulator of a variety of physiological processes, such as cell differentiation, proliferation and apoptosis. Here we showed that Egr2 was downregulated by bone morphogenetic protein (BMP) signaling pathways during the commitment of C3H10T1/2 stem cells to adipocyte lineage. Overexpression of Egr2 completely prevented BMP4-induced adipocyte lineage commitment of C3H10T1/2 stem cells, while simultaneously stimulating early smooth muscle-like differentiation. We also demonstrated that Egr2-induced early smooth muscle-like differentiation is transforming growth factor β1-independent. Egr2 can activate the transcription of early smooth muscle cell specific genes smooth muscle protein 22α and calponin 1. Together, the results indicated a novel role for Egr2 in repressing adipocyte lineage commitment and promoting early smooth muscle-like cell differentiation.

Copyright © 2013 Elsevier Ltd. All rights reserved.

KEYWORDS:

422/aP2; Adipogenesis; BMP4; C3H10T1/2; Cnn1; Commitment; Egr1; Egr2; MSC; Myocd; PPARγ; SMC; Sm22α; Sm22β; Smooth muscle cell; Srf; Tgfβ1; actin alpha 2 smooth muscle aorta; bone morphogenetic protein 4; calponin 1; early growth response 1; early growth response 2; fatty acid-binding protein; mesenchymal stem cell; myocardin; peroxisome proliferator-activated receptors γ; serum response factor; smooth muscle cell; smooth muscle protein 22α; smooth muscle protein 22β; transforming growth factor β1; α-SMA

PMID:
23751188
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk