Send to:

Choose Destination
See comment in PubMed Commons below
Springerplus. 2013 Mar 9;2(1):93. doi: 10.1186/2193-1801-2-93. Print 2013 Dec.

Rhodomycin analogues from Streptomyces purpurascens: isolation, characterization and biological activities.

Author information

  • 1Department of Microbiology, School of Life Sciences, Swami Ramanand Teerth Marathwada University (SRTMU), Nanded, 431 401 Maharashtra India.


During a screening program for bioactive natural products, a potential Streptomyces sp was isolated from soil. On the basis of biochemical, cultural, physiological and 16S rRNA gene analysis, it was identified as Streptomyces purpurascens. The isolate was grown in liquid medium and the crude antibiotic complex was obtained by ethyl acetate extraction. Seven purified fractions were obtained by preparative Thin Layer Chromatography (TLC). Acid hydrolysis of each fraction and subsequent TLC led to the identification of aglycones and sugars indicating these compounds to be Rhodomycin and its analogues. The identity of these compounds was established on the basis of UV-visible and FT-IR spectra and comparison with published data. The compounds were active against Gram-positive bacteria. Compound E, identified as Rhodomycin B, was found to be the most potent compound with an MIC of 2 μg/ml against Bacillus subtilis. Compounds A and F identified as α2-Rhodomycin II and Obelmycin respectively, and Compound E exhibited an IC50 of 8.8 μg/ml against HeLa cell line but no cytotoxicity was found against L929.


16S rRNA; Bioactivity; Rhodomycin; Streptomyces purpurascens; Taxonomy

Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Icon for PubMed Central
    Loading ...
    Write to the Help Desk