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Placenta. 2013 Aug;34(8):722-5. doi: 10.1016/j.placenta.2013.05.001. Epub 2013 May 31.

Memb-roller: an effective way of making membrane rolls for pathological examination and studies of human placenta.

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  • 1Asan Laboratory of Perinatal Science, Asan Institute for Life Sciences, Asan Medical Center, Seoul, Republic of Korea.

Abstract

For the pathological evaluation of the chorioamniotic membranes, a membrane roll is made to give an orientation to the sections and to cover more extended planes. We designed a new instrument, Annie's memb-roller, for easy preparation of membrane rolls. Annie's memb-roller has two non-toothed arms which are 2 mm wide, 1 mm thick, and 8 cm long. To test its effectiveness in making a membrane roll, the placentas obtained from pregnant women (n = 10) with various obstetrical conditions were used. Preparation of the membrane rolls using Annie's memb-roller was easy and successful in all cases tested. The lengths of the membranes measured from fresh placentas and paraffin sections showed a very good correlation (p < 0.001; r = 0.941). Application of the memb-roller would be very helpful in the semi-quantitative analysis of the chorioamniotic membranes and the standardization of placental pathology and research practice.

Copyright © 2013 Elsevier Ltd. All rights reserved.

PMID:
23731798
[PubMed - indexed for MEDLINE]
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