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J Fish Biol. 2013 Jun;82(6):1888-903. doi: 10.1111/jfb.12116. Epub 2013 Apr 26.

Characterization of a novel cell line from the caudal fin of koi carp Cyprinus carpio.

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  • 1Institute of Fisheries Science, National Taiwan University, Taipei, Taiwan, China.

Abstract

A continuous cell line (KF-101) derived from the caudal fin of the koi carp Cyprinus carpio was established and characterized. The KF-101 cell line multiplied abundantly in Leibovitz's L-15 medium containing 10% foetal bovine serum at 25° C, and was subcultured for >90 passages over a period of 3 years. Immunocytochemistry revealed that the KF-101 cells contain keratin, junction proteins connexin-43 and occludin, and ectodermal stem-cell marker Pax-6, but not vimentin. Furthermore, the KF-101 cells reacted with anti-human DARPP-32 and anti-human GATA-4 antibodies, and the labelling was regulated according to the cell cycle. The labels of the DARPP-32 and GATA-4 antibodies in the KF-101 cells were the suggested phosphatase-1 inhibitor-1 and GATA-3, respectively. In addition, the KF-101 cells were susceptible to koi herpesvirus but were resistant to eel herpesvirus, iridovirus, grouper nodavirus and chum salmon (Oncorhynchus keta) virus. The results indicate that the KF-101 cells are suitable materials for investigating biological and virological development.

© 2013 The Authors. Journal of Fish Biology © 2013 The Fisheries Society of the British Isles.

[PubMed - indexed for MEDLINE]
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