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Front Behav Neurosci. 2013 May 22;7:51. doi: 10.3389/fnbeh.2013.00051. eCollection 2013.

Egr-1 induction provides a genetic response to food aversion in zebrafish.

Author information

  • 1INSERM U 1024, CNRS UMR 8197, Ecole Normale Supérieure, IBENS, Developmental Biology Paris, France.

Abstract

As soon as zebrafish larvae start eating, they exhibit a marked aversion for bitter and acidic substances, as revealed by a consumption assay, in which fluorescent Tetrahymena serve as a feeding basis, to which various stimuli can be added. Bitter and acidic substances elicited an increase in mRNA accumulation of the immediate-early response gene egr-1, as revealed by in situ hybridization. Conversely, chemostimulants that did not induce aversion did not induce egr-1 response. Maximum labeling was observed in cells located in the oropharyngeal cavity and on the gill rakers. Gustatory areas of the brain were also labeled. Interestingly, when bitter tastants were repeatedly associated with food reward, zebrafish juveniles learned to ingest food in the presence of the bitter compound. After habituation, the acquisition of acceptance for bitterness was accompanied by a loss of egr-1 labeling. Altogether, our data indicate that egr-1 participates specifically in food aversion. The existence of reward-coupled changes in taste sensitivity in humans suggests that our results are relevant to situations in humans.

KEYWORDS:

bitterness; consumption assay; egr-1; taste aversion; zebrafish

PMID:
23720615
[PubMed]
PMCID:
PMC3660967
Free PMC Article
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