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Asian Pac J Trop Med. 2013 Jun;6(6):473-80. doi: 10.1016/S1995-7645(13)60077-2.

Identification of target genes of transcription factor CEBPB in acute promyelocytic leukemia cells induced by all-trans retinoic acid.

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  • 1Department of Hepatic Surgery, National Hepatobiliary and Enteric Surgery Research Center, Ministry of Health, Central South University, China.

Abstract

OBJECTIVE:

To identify target genes of transcription factor CCAAT enhancer-binding protein β (CEBPB) in acute promyelocytic leukemia cells induced by all-trans retinoic acid.

METHODS:

A new strategy for high-throughput identification of direct target genes was established by combining chromatin immunoprecipitation (ChIP) with in vitro selection. Then, 106 potential CEBPB binding fragments from the genome of the all-trans retinoic acid (ATRA)-treated NB4 cells were identified.

RESULTS:

Of them, 82 were mapped in proximity to known or previously predicted genes; 7 were randomly picked up for further confirmation by ChIP-PCR and 3 genes (GALM, ITPR2 and ORM2) were found to be specifically up-regulated in the ATRA-treated NB4 cells, indicating that they might be the down-stream target genes of ATRA.

CONCLUSIONS:

Our results provided new insight into the mechanisms of ATRA-induced granulocytic differentiation.

Copyright © 2013 Hainan Medical College. Published by Elsevier B.V. All rights reserved.

PMID:
23711709
[PubMed - indexed for MEDLINE]
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