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Comput Math Methods Med. 2013;2013:653759. doi: 10.1155/2013/653759. Epub 2013 Apr 28.

Uses of phage display in agriculture: a review of food-related protein-protein interactions discovered by biopanning over diverse baits.

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  • 1Department of Horticulture, Agricultural Science Center North, University of Kentucky, Room 308J, Lexington, KY 40546, USA ; Seed Biology Group, University of Kentucky, Lexington, KY 40546, USA.

Abstract

This review highlights discoveries made using phage display that impact the use of agricultural products. The contribution phage display made to our fundamental understanding of how various protective molecules serve to safeguard plants and seeds from herbivores and microbes is discussed. The utility of phage display for directed evolution of enzymes with enhanced capacities to degrade the complex polymers of the cell wall into molecules useful for biofuel production is surveyed. Food allergies are often directed against components of seeds; this review emphasizes how phage display has been employed to determine the seed component(s) contributing most to the allergenic reaction and how it has played a central role in novel approaches to mitigate patient response. Finally, an overview of the use of phage display in identifying the mature seed proteome protection and repair mechanisms is provided. The identification of specific classes of proteins preferentially bound by such protection and repair proteins leads to hypotheses concerning the importance of safeguarding the translational apparatus from damage during seed quiescence and environmental perturbations during germination. These examples, it is hoped, will spur the use of phage display in future plant science examining protein-ligand interactions.

PMID:
23710253
[PubMed - indexed for MEDLINE]
PMCID:
PMC3655605
Free PMC Article

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